Southampton FC's B team will start their 2021/22 Papa John's trophy campaign with a trip to League Two side Leyton Orient in mid-September.
Dave Horseman's side will remain in London for their second game of Group G, travelling south east to The Valley as League One side Charlton Athletic host in early October.
Their final group game will see Southampton's youngsters face League Two side Crawley Town, visiting The People's Pension Stadium at the start of November.
Ticket details for the games will be released in due course.
Leyton Orient vs Saints
Tuesday 14th September, Breyer Group Stadium, 7pm BST
Charlton Athletic vs Saints
Tuesday 5th October, The Valley, 7pm BST
Crawley Town vs Saints
Tuesday 9th November, The People's Pension Stadium, 7pm GMT